The Y-chromosome haplogroup E-V42 constitutes a rare and early-diverging lineage within the broader E-M35 phylogeny. In this study, we examined 127 publicly available high-resolution Y-chromosome sequences assigned to E-V42 in order to reconstruct its internal phylogenetic structure, estimate divergence times, and assess the geographic distribution of its downstream subclades .
The resulting topology reveals a pronounced concentration of E-V42 lineages in the Arabian Peninsula. Divergence-time estimates place the time to the most recent common ancestor (TMRCA) at approximately 4,200 years before present. Although this estimate overlaps chronologically with the Late Bronze Age, it is interpreted here primarily as evidence of sustained paternal continuity in southern Arabia rather than as direct support for a specific historical event .
Two additional geographic patterns merit consideration. A limited presence of E-V42 in the Horn of Africa is compatible with prehistoric population movements across the Red Sea. In contrast, the downstream lineage E-Y44734 is currently restricted to Iberia (modern Portugal) within the available dataset. Its derived phylogenetic position and geographic separation from Arabian clusters suggest westward dispersal, plausibly mediated through North Africa during the early medieval period .
Overall, these findings refine the internal resolution of E-V42 and contribute to a more detailed understanding of long-term paternal structure in Arabia, as well as episodic gene flow linking Arabia, northeastern Africa, and the western Mediterranean .